Coliform Bacteria in drinking water Hydrogen Sulfide

The detection of certain types of coliform bacteria in drinking water, such as Citrobacter and some harmful bacteria like Salmonella and Proteus which are all hydrogen sulfide producing bacteria which cause diarrhea, can be done by using field test kit, V810 Medium (culture media absorbed in cotton ball). Using V 810 Medium to detect those hydrogen sulfide producing bacteria in drinking water is very simple, easy and convenient. If the water is contaminated, the culture medium will change from its original clear light yellow to slightly black, black and more black precipitate depending on the degree of contamination. Also the presence of emergence of gas when shaken the reaction bottle gently confirms positive hydrogen sulfide producing bacteria contamination

World Health Organization (WHO) Guidelines for drinking water quality in the Year of 1993

Various methods for management of drinking water which contaminated by bacteria

Package: 20 Test

Storage: 4-25 C Stability: 12 Month

Precaution: Keep away from children, Sunlight, Moisture and Heat

1. Boiling : Boil water at 100° C at least 5 minutes and keep it in clean and closed appropriate container
2. Using Chemicals: Using effective chlorine gas or chlorine powder.(free residual Chlorine in the drinking water after treatment is 0.2-0.5 ppm.)
3. Filtration: Trap bacteria by using certain effective filtrator.
4. Treatment by using UV light or ozone.

Detection of Hydrogen Sulfide Producing Bacteria in Drinking

Bottle 1 : culture cotton ball in bottle prior to adding water sample

Bottle 2 : immediately after adding 20 ml water sample up to mark 4 on the bottle

Bottle 3 : after keeping in room temperature (25-40 degree Celsius) for 24-48 hours.

: this Colour (clear light yellow) indicates no harmful bacteria (-) in water sample are detected

: the water is safe for human consumption

Bottle 4 : after keeping in room temperature (25-40 degree Celsius) for 24-48 hours.

: this colour (slightly black precipitate, turbidity ( ), little rotten egg smell) indicates positive harmful bacteria ( ) in water sample

: the water is not safe for human consumption

Bottle 5 : after keeping in room temperature (25-40 degree Celsius) for 24-48 hours

: this colour (more black precipitate, turbidity ( ), rotten egg smell) indicates positive harmful bacteria ( ) in water sample

: the water is not safe for human consumption

Bottle 6 : after keeping in room temperature (25-40 degree Celsius) for 24-48 hours

: this colour (black precipitate, turbidity ( ), rotten egg smell) indicates heavy harmful bacteria ( ) in water sample

: the water is not safe for human consumption