the GFast Test kit is a qualitative screen, colourimetic assay for the detection of organophosphate and carbamate pesticide residues in vegetables and fruits, cereals.
based on their inhibition of the enzyme cholinesterase (ChE). If pesticides from the organophosphate and carbamate groups are present, color formation will be reduced.
Detection limit for ability to inhibit the enzyme is 15% which means that slight poisoning indicates unsafe. Accuracy and specificity of procedure are 85 and 81%, respectively (false positive is 15%, false negative is 0%)
The GFast screening Kit provides screening results
based on their inhibition of the enzyme cholinesterase (ChE). If pesticides from the organophosphate and carbamate groups are present, color formation will be reduced.
Detection limit for ability to inhibit the enzyme is 15% which means that slight poisoning indicates unsafe. Accuracy and specificity of procedure are 85 and 81%, respectively (false positive is 15%, false negative is 0%)
Number of Sample Test Kit: 10 Samples/Kit
The Sample: Vegetable, Fruits and Cereals
Stability: 12 month
Test Kit Component
Materials
- Plastic bottle (extract bottle) 10 bottles
- Glass test tube 10 tubes
- Plastic test tube 11 tubes
- 3 cc. Plastic dropper 3 pieces
- Small plastic dropper 1 pieces
- Gloves 2 pairs 1 piece
Solutions
- Extract solution 1 bottle
- Distilled water 1 bottle
- Solution 1 1 bottle
- Solution 2 1 bottle
- Solution 3 1 bottle
Interpretation of Results
Evaluate the develop colour of sample tube compare to negative control tube.
Dark orange as control tube–Safe
Orange mix with pink –Unsafe (15 % inhibition)
Light pink–strongly unsafe
Quality Control
The negative control must be included in each ruin and must use the same reagents used with the unknown samples. If the negative control fails to result observable dark orange colour, the test is invalid and should be repeated.
The advantages of the GFast pesticides test kit it using without equipment or laboratory facilities used onsite and at field farm.
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