The principle of the GT-Test kit is based on measuring the inhibition of the enzyme cholinesterase. Some pesticides e.g. organophosphorus and carbamates inhibit this enzyme. The GT-test kit is capable of detecting whether or not substances in the sample inhibit the enzyme.
The GT-test kit is very easy to use and can be carried out anywhere e.g. on markets. The results are produced very rapid (few hours) and at a very low price. No expensive instruments are used.
Pesticides Rapid test kit (carbamate and Organophosphate group) Pesticides and insecticides are poisonous agents limited for agricultural use only. Target sample fresh vegetable and fruit, salted fish
Health Impact
consumption of food contained pesticide residue can cause weakness, low immunity. Obtaining high concentration of pesticides can cause dizziness, vomiting, dyspnea, and cardiac arrest.
Effectiveness of Test Kit
To detection of toxic residue Phosphate and carbamate groups organochlorina, Synthetic pyrethroid, Cholinesterase inhibitors reagent in food within 30-60 minutes
Detection limit 0.05 mg./Kg. The sensitive standard EU and Japan
As Trichlorfon = 0.05 mg/kg
Value of Inhibition 50% as Trichlorfon = 0.2 mg/kg
Vegetables Dry salted fish
Sensitivity 92.3 92.7%
Specificity 85.1% 77.5%
Accuracy 87.1% 83.0%
Positive prediction value 70.6% 65.5%
Negative prediction value 96.6% 95.8%
GT Pesticides Detection Kit (Code: AP001)
Pesticides are toxic substances to be used in agricultural products residues need to be lower than the safety limit there are still many kinds of food in the market that still has residues that excess the safety limit. Several methods can be applied to detect pesticides residue in food samples, such as GC, HPLC and GCMS. Those methods require a long analysis time as well as complicated procedures and instruments. This Sensitive pesticides Detection kit provides a fast, Easy, Simple and reliable test solutions to detect pesticides residues in food samples.
Pesticides Test targeted groups
- Organophosphate group
- Carbamate group
- Cholinesterase Inhibitors
Target samples:
Vegetables, Fruits, cereal grains, medicinal plants, dry salted fish, soil, mud and water from/near contaminate area and drinking water
Details:
This kit is composed of 2 parts
- Test kit (reagents only) 10 test and 30 test/Kit
- Modified equipment (you do not need this if you have access to laboratory equipment)
- 1 unit of modified warm water bath
- 1 unit of Thermometer
- 18 test tubes
- 5 Pasture pipettes
- 12 plastic pipettes
- Aquatic air pump
- 1 unit of evaporated kit
Extraction Procedure
- Chop or blend sample
- Weight 5 g of the homogenous sample in to the sample bottle
- Add 5mL of Solvent-1 into the sample bottle, closed tight and shake vigorously for approximately 1 minute. Let is stand for 10-15 minutes
- Pipette out 1mL of Solvent -2
- Now, prepare the modified warm water bath (32-36C) by connecting the evaporated kit with the air pump and the pasture pipette and then inset the end of the pasture from the air pump in to the extract, and then incubate until the Solvent-1 (lower layer) is evaporated now the solution is called “Sample Extract”
Testing Procedure
Prepare and label at least 3 test tubes for:
- Tube 1: Cut point or unsafe
- Tube 2: Control or not detected
- Tube 3: Sample extract (sample name}
3 test tubes for 1 sample. Please add more test tubes if you are testing more samples. Run each samples with two control tubes (Tube 1 and 2)
- Add 0.25mL of Solvent-2 into Tube 1and 2
- Add 0.25mL of each Sample Extract from the extraction procedure to Tube 3 or your sample tubes
- Put all the tubes into the modified warm water bath at temperature (32-36C)
- Pipette each 0.5nL of Marin -1 solution into every tubes and incubate for 5-10 minutes
- During incubation mix Main-2 solution: Main-2+Main 2.1 and Main-3 solution: Main -3+Main 3.1
- Add 0.375mL of the mixed Main-2 solutions from step 5 into Tube-1 (cut point of unsafe control tube). Add0.25mL of the Main-2 solution in step 5 into Tube 2 (safe contril tube) and Tube
- (sample tube) incubate for 30 minutes
- Add 1 mL of main -4 solutions from step 5 into every tube and swirl
- Add 0.5mL of Main 5 solution into every tube and swirl
- Evaluate the results
Color in the tube |
Result Interpretation |
Sample tube <tube2 |
Not detected |
Sample tube > tube 2 but< tube1 |
There are some toxic residues expected safe for consumption |
Sample tube > tube 2 and > tube 1 |
There are some toxic residues expected unsafe *** for consumption |
Note:
Safe for consumption = there are some toxic residues inhibited the cholinesterase enzyme at less than 50% these toxic residues amounts can be washed out by the consumer
Unsafe for consumption = there are some toxic residues inhibited the cholinesterase enzyme at 50% or more. This amount of toxic residue cannot be washed out by the consumer, they are only decreased by washing
CAUTION
- The reason in using the Solvent-1 are to dissolve the toxic residues from the sample and to destroy the interfering enzyme deposited in the sample. Therefore Solvent -1 is toxic for human health, avoid breathing and leave it in open, as it will evaporate
- Wash out with clean water and soap when chemical reagents in contact with skin
- Wear gloves
- Keep away from children
- Do not use reagents from different are chemical
- This not food DO NOT eat or drink
Storage
- Keep all reagents in a refrigerator/Cool place Except for Main-GT1 and Main GT2 should be in the freezer
- 1 year shelf life if stored properly
- Once Main GT2, Main-GT2.1, Main-GT3.1 are mixed, store in the refrigerator
- Main-GT2 mixture can be used within 10 days after mixing
- Main-GT3 mixture can be used within 3-4 days after mixing
instruction click here: https://asianmedicstore.com/wp-content/uploads/2019/06/GT-Pesticides-in-food.pdf
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